Paper-based Microfluidic Device for Norovirus Detection

Aptamer-based Fluorometric Determination of Norovirus Using a Paper-based Microfluidic Device

Xuan Weng & Suresh Neethirajan

Microchimica Acta DOI: 10.1007/s00604-017-2467-x  (Link)

 

We describe a rapid and highly sensitive biosensor towards point-of-care device for rapid determination of noroviruses, a leading cause of outbreak of acute gastroenteritis worldwide. It is based on the use of a norovirus-specific aptamer labeled with 6-carboxyfluorescein, and of multi-walled carbon nanotubes (MWCNT) and graphene oxide (GO). The fluorescence of the 6-FAM labeled aptamer is quenched by MWCNT or GO via fluorescence resonance energy transfer (FRET). In the presence of norovirus, fluorescence is recovered due to the release of the labeled aptamer from MWCNT or GO. An easy-to-make paper-based microfluidic platform was developed using a nitrocellulose membrane. The quantitation of norovirus was successfully performed. The linear range extends from 12.9 ng⋅mL⁻¹ ~12.9 µg⋅mL⁻¹ of norovirus. The detection limits are 4.4 ng⋅mL⁻¹ and 3.3 ng⋅mL⁻¹, respectively, when using MWCNT or GO. The device is simple and cost-effective, and holds the potential of rapid in-situ visual determination of noroviruses with remarkable sensitivity and specificity, which provides a new method for early identification of norovirus and thereby an early intervention for preventing the spread of an outbreak.

Keywords: Biosensor; Aptamer; Norovirus; Paper-based microfluidic device; Nitrocellulose membrane; Multi-walled carbon nanotubes; Graphene oxide

 

 

 

Contact Us

Bionanotechnology Laboratory
Suresh Neethirajan

School of Engineering
University of Guelph
Guelph, Ontario
Canada N1G 2W1

Office:
Room 3513 - Richards Building
50 Stone Road East

Lab: THRN 2133 BioNano Lab

Phone: (519) 824-4120 Ext 53922
Fax: (519) 836-0227

E-mail: sneethir@uoguelph.ca

 
© 2016 Bionano Lab - University of Guelph. All Rights Reserved.