Lab News

Suresh presented a paper on '3D Imaging and Morphological Study of the Internal Structure of Cereal Grains Using X-ray Micro Computed Tomography' at the 46th ISAE Convention and International Grain Storage Symposium, Pant Nagar - India on Feb 28, 2012.

Internal structure of cereal grains were investigated using three-dimensional X-ray micro computed tomography images. Reconstruction of numerical samples of grain kernels aided in understanding the distribution of starch matrix and the intercellular path networks. Three dimensional quantitative analysis of internal structure of insect infested wheat kernel, sprout damaged durum wheat kernel, canola, corn and barley kernel revealed the inter-relationships between the structure and function. Stereological analyses using the developed algorithms helped to determine the void volume fraction, specific surface area and the connectivity between the tissues. The results suggests that the combination of computed tomography with image processing delivers geometric parameters of pore structure inside single cereal grain kernels for analysing and developing moisture diffusion models.

Keywords:  X-ray Micro CT; Image Processing; Grain Storage; Grain Geometry; Moisture Diffusivity Schedule fi.pdf

Suresh delivered an invited talk at the 1st Biotechnology World Congress, at Dubai, February 14-15, 2012. Neethirajan.htm

Bionanotechnology - Emerging Trends for Agriculture, Food and Biosystems

Bionanotechnology is an emerging interdisciplinary field at the interface of biotechnology and nanotechnology. Being in the incipient stage, the topical areas of bionanotechnology research are in a wide spectrum. Nanoscale technology is well suited for understanding the interfaces between organisms in systems biology. The challenges associated in characterizing the diverse organisms involved in the plant-microbe interface and dissecting their molecular-based exchanges are being currently addressed by our developed analytical tools and devices. Microfluidic devices combined with nanoscale features aids in functionally assaying bacteria in investigating the species variation during motility. Imaging studies of colonization and surface adhesion kinetics of bacteria using confocal fluorescence and atomic force microscopy (AFM) reveals the evolution of distinct microbial biofilm morphologies and the ultra structure of the bacterial pili. Nonintrusive investigation of single biomolecules is challenging but useful for screening and diagnostic purposes as there are connections between biomarkers and genetic disorders. An accurate methodology for karyotyping of chromosomes and fabrication of DNA nanowire templates are being discussed. This presentation will highlight the uses of micro and nanotechnologies that have been developed for studying biomacromolecules as well as understanding the various aspects of plant-microbe interface.

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Bionanotechnology Laboratory
Suresh Neethirajan

School of Engineering
University of Guelph
Guelph, Ontario
Canada N1G 2W1

Room 3513 - Richards Building
50 Stone Road East

Lab: THRN 2133 BioNano Lab

Phone: (519) 824-4120 Ext 53922
Fax: (519) 836-0227


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